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Tracking of enzymatic biomass deconstruction by fungal secretomes highlights markers of lignocellulose recalcitrance.

Identifieur interne : 000659 ( Main/Exploration ); précédent : 000658; suivant : 000660

Tracking of enzymatic biomass deconstruction by fungal secretomes highlights markers of lignocellulose recalcitrance.

Auteurs : Gabriel Paës [France] ; David Navarro [France] ; Yves Benoit [France] ; Senta Blanquet [France] ; Brigitte Chabbert [France] ; Bernard Chaussepied [France] ; Pedro M. Coutinho [France] ; Sylvie Durand [France] ; Igor V. Grigoriev [États-Unis] ; Mireille Haon [France] ; Laurent Heux [France] ; Charlène Launay [France] ; Antoine Margeot [France] ; Yoshiharu Nishiyama [France] ; Sana Raouche [France] ; Marie-Noëlle Rosso [France] ; Estelle Bonnin [France] ; Jean-Guy Berrin [France]

Source :

RBID : pubmed:30976326

Abstract

Background

Lignocellulose biomass is known as a recalcitrant material towards enzymatic hydrolysis, increasing the process cost in biorefinery. In nature, filamentous fungi naturally degrade lignocellulose, using an arsenal of hydrolytic and oxidative enzymes. Assessment of enzyme hydrolysis efficiency generally relies on the yield of glucose for a given biomass. To better understand the markers governing recalcitrance to enzymatic degradation, there is a need to enlarge the set of parameters followed during deconstruction.

Results

Industrially-pretreated biomass feedstocks from wheat straw, miscanthus and poplar were sequentially hydrolysed following two steps. First, standard secretome from

Conclusions

Fungal secretomes applied to highly recalcitrant biomass samples can further extend the release of the remaining glucose. The glucose yield can be correlated to chemical and physical markers, which appear to be independent from the biomass type and secretome. Overall, correlations between these markers reveal how nano-scale properties (polymer content and organization) influence macro-scale properties (particle size and water sorption). Further systematic assessment of these markers during enzymatic degradation will foster the development of novel cocktails to unlock the degradation of lignocellulose biomass.


DOI: 10.1186/s13068-019-1417-8
PubMed: 30976326
PubMed Central: PMC6442405


Affiliations:

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<name sortKey="Raouche, Sana" sort="Raouche, Sana" uniqKey="Raouche S" first="Sana" last="Raouche">Sana Raouche</name>
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<name sortKey="Rosso, Marie Noelle" sort="Rosso, Marie Noelle" uniqKey="Rosso M" first="Marie-Noëlle" last="Rosso">Marie-Noëlle Rosso</name>
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<name sortKey="Bonnin, Estelle" sort="Bonnin, Estelle" uniqKey="Bonnin E" first="Estelle" last="Bonnin">Estelle Bonnin</name>
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<region type="region">Pays de la Loire</region>
<region type="old region">Pays de la Loire</region>
<settlement type="city">Nantes</settlement>
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<author>
<name sortKey="Berrin, Jean Guy" sort="Berrin, Jean Guy" uniqKey="Berrin J" first="Jean-Guy" last="Berrin">Jean-Guy Berrin</name>
<affiliation wicri:level="3">
<nlm:affiliation>2INRA, Aix Marseille Univ., UMR1163, BBF, Biodiversité et Biotechnologie Fongiques, Marseille, France.</nlm:affiliation>
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<region type="region">Provence-Alpes-Côte d'Azur</region>
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<series>
<title level="j">Biotechnology for biofuels</title>
<idno type="ISSN">1754-6834</idno>
<imprint>
<date when="2019" type="published">2019</date>
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<front>
<div type="abstract" xml:lang="en">
<p>
<b>Background</b>
</p>
<p>Lignocellulose biomass is known as a recalcitrant material towards enzymatic hydrolysis, increasing the process cost in biorefinery. In nature, filamentous fungi naturally degrade lignocellulose, using an arsenal of hydrolytic and oxidative enzymes. Assessment of enzyme hydrolysis efficiency generally relies on the yield of glucose for a given biomass. To better understand the markers governing recalcitrance to enzymatic degradation, there is a need to enlarge the set of parameters followed during deconstruction.</p>
</div>
<div type="abstract" xml:lang="en">
<p>
<b>Results</b>
</p>
<p>Industrially-pretreated biomass feedstocks from wheat straw, miscanthus and poplar were sequentially hydrolysed following two steps. First, standard secretome from </p>
</div>
<div type="abstract" xml:lang="en">
<p>
<b>Conclusions</b>
</p>
<p>Fungal secretomes applied to highly recalcitrant biomass samples can further extend the release of the remaining glucose. The glucose yield can be correlated to chemical and physical markers, which appear to be independent from the biomass type and secretome. Overall, correlations between these markers reveal how nano-scale properties (polymer content and organization) influence macro-scale properties (particle size and water sorption). Further systematic assessment of these markers during enzymatic degradation will foster the development of novel cocktails to unlock the degradation of lignocellulose biomass.</p>
</div>
</front>
</TEI>
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<PMID Version="1">30976326</PMID>
<DateRevised>
<Year>2020</Year>
<Month>09</Month>
<Day>30</Day>
</DateRevised>
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<Journal>
<ISSN IssnType="Print">1754-6834</ISSN>
<JournalIssue CitedMedium="Print">
<Volume>12</Volume>
<PubDate>
<Year>2019</Year>
</PubDate>
</JournalIssue>
<Title>Biotechnology for biofuels</Title>
<ISOAbbreviation>Biotechnol Biofuels</ISOAbbreviation>
</Journal>
<ArticleTitle>Tracking of enzymatic biomass deconstruction by fungal secretomes highlights markers of lignocellulose recalcitrance.</ArticleTitle>
<Pagination>
<MedlinePgn>76</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1186/s13068-019-1417-8</ELocationID>
<Abstract>
<AbstractText Label="Background" NlmCategory="UNASSIGNED">Lignocellulose biomass is known as a recalcitrant material towards enzymatic hydrolysis, increasing the process cost in biorefinery. In nature, filamentous fungi naturally degrade lignocellulose, using an arsenal of hydrolytic and oxidative enzymes. Assessment of enzyme hydrolysis efficiency generally relies on the yield of glucose for a given biomass. To better understand the markers governing recalcitrance to enzymatic degradation, there is a need to enlarge the set of parameters followed during deconstruction.</AbstractText>
<AbstractText Label="Results" NlmCategory="UNASSIGNED">Industrially-pretreated biomass feedstocks from wheat straw, miscanthus and poplar were sequentially hydrolysed following two steps. First, standard secretome from
<i>Trichoderma reesei</i>
was used to maximize cellulose hydrolysis, producing three recalcitrant lignin-enriched solid substrates. Then fungal secretomes from three basidiomycete saprotrophs (
<i>Laetisaria arvalis, Artolenzites elegans</i>
and
<i>Trametes ljubarskyi</i>
) displaying various hydrolytic and oxidative enzymatic profiles were applied to these recalcitrant substrates, and compared to the
<i>T. reesei</i>
secretome. As a result, most of the glucose was released after the first hydrolysis step. After the second hydrolysis step, half of the remaining glucose amount was released. Overall, glucose yield after the two sequential hydrolyses was more dependent on the biomass source than on the fungal secretomes enzymatic profile. Solid residues obtained after the two hydrolysis steps were characterized using complementary methodologies. Correlation analysis of several physico-chemical parameters showed that released glucose yield was negatively correlated with lignin content and cellulose crystallinity while positively correlated with xylose content and water sorption. Water sorption appears as a pivotal marker of the recalcitrance as it reflects chemical and structural properties of lignocellulosic biomass.</AbstractText>
<AbstractText Label="Conclusions" NlmCategory="UNASSIGNED">Fungal secretomes applied to highly recalcitrant biomass samples can further extend the release of the remaining glucose. The glucose yield can be correlated to chemical and physical markers, which appear to be independent from the biomass type and secretome. Overall, correlations between these markers reveal how nano-scale properties (polymer content and organization) influence macro-scale properties (particle size and water sorption). Further systematic assessment of these markers during enzymatic degradation will foster the development of novel cocktails to unlock the degradation of lignocellulose biomass.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y">
<Author ValidYN="Y">
<LastName>Paës</LastName>
<ForeName>Gabriel</ForeName>
<Initials>G</Initials>
<AffiliationInfo>
<Affiliation>1FARE Laboratory, INRA, Université de Reims Champagne-Ardenne, Reims, France.</Affiliation>
<Identifier Source="ISNI">0000 0004 1937 0618</Identifier>
<Identifier Source="GRID">grid.11667.37</Identifier>
</AffiliationInfo>
</Author>
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<LastName>Navarro</LastName>
<ForeName>David</ForeName>
<Initials>D</Initials>
<AffiliationInfo>
<Affiliation>2INRA, Aix Marseille Univ., UMR1163, BBF, Biodiversité et Biotechnologie Fongiques, Marseille, France.</Affiliation>
<Identifier Source="GRID">grid.503114.2</Identifier>
</AffiliationInfo>
<AffiliationInfo>
<Affiliation>3INRA, Aix-Marseille Univ., UMR1163, CIRM-CF, Marseille, France.</Affiliation>
<Identifier Source="ISNI">0000 0001 2176 4817</Identifier>
<Identifier Source="GRID">grid.5399.6</Identifier>
</AffiliationInfo>
</Author>
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<LastName>Benoit</LastName>
<ForeName>Yves</ForeName>
<Initials>Y</Initials>
<AffiliationInfo>
<Affiliation>4IFP Energies Nouvelles, Rueil-Malmaison, France.</Affiliation>
<Identifier Source="ISNI">0000 0001 2159 7561</Identifier>
<Identifier Source="GRID">grid.13464.34</Identifier>
</AffiliationInfo>
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<LastName>Blanquet</LastName>
<ForeName>Senta</ForeName>
<Initials>S</Initials>
<AffiliationInfo>
<Affiliation>4IFP Energies Nouvelles, Rueil-Malmaison, France.</Affiliation>
<Identifier Source="ISNI">0000 0001 2159 7561</Identifier>
<Identifier Source="GRID">grid.13464.34</Identifier>
</AffiliationInfo>
</Author>
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<LastName>Chabbert</LastName>
<ForeName>Brigitte</ForeName>
<Initials>B</Initials>
<AffiliationInfo>
<Affiliation>1FARE Laboratory, INRA, Université de Reims Champagne-Ardenne, Reims, France.</Affiliation>
<Identifier Source="ISNI">0000 0004 1937 0618</Identifier>
<Identifier Source="GRID">grid.11667.37</Identifier>
</AffiliationInfo>
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<LastName>Chaussepied</LastName>
<ForeName>Bernard</ForeName>
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<AffiliationInfo>
<Affiliation>4IFP Energies Nouvelles, Rueil-Malmaison, France.</Affiliation>
<Identifier Source="ISNI">0000 0001 2159 7561</Identifier>
<Identifier Source="GRID">grid.13464.34</Identifier>
</AffiliationInfo>
</Author>
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<LastName>Coutinho</LastName>
<ForeName>Pedro M</ForeName>
<Initials>PM</Initials>
<AffiliationInfo>
<Affiliation>5CNRS, Aix-Marseille Univ., UMR7857 AFMB, Architecture et Fonction des Macromolécules Biologiques, Marseille, France.</Affiliation>
<Identifier Source="ISNI">0000 0004 1798 275X</Identifier>
<Identifier Source="GRID">grid.463764.4</Identifier>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Durand</LastName>
<ForeName>Sylvie</ForeName>
<Initials>S</Initials>
<AffiliationInfo>
<Affiliation>6INRA, UR1268 Biopolymères Interactions Assemblages, Nantes, France.</Affiliation>
<Identifier Source="GRID">grid.460203.3</Identifier>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Grigoriev</LastName>
<ForeName>Igor V</ForeName>
<Initials>IV</Initials>
<AffiliationInfo>
<Affiliation>7US Department of Energy Joint Genome Institute, Walnut Creek, CA USA.</Affiliation>
<Identifier Source="ISNI">0000 0004 0449 479X</Identifier>
<Identifier Source="GRID">grid.451309.a</Identifier>
</AffiliationInfo>
<AffiliationInfo>
<Affiliation>8Department of Plant and Microbial Biology, University of California Berkeley, Berkeley, CA USA.</Affiliation>
<Identifier Source="ISNI">0000 0001 2181 7878</Identifier>
<Identifier Source="GRID">grid.47840.3f</Identifier>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Haon</LastName>
<ForeName>Mireille</ForeName>
<Initials>M</Initials>
<AffiliationInfo>
<Affiliation>2INRA, Aix Marseille Univ., UMR1163, BBF, Biodiversité et Biotechnologie Fongiques, Marseille, France.</Affiliation>
<Identifier Source="GRID">grid.503114.2</Identifier>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Heux</LastName>
<ForeName>Laurent</ForeName>
<Initials>L</Initials>
<AffiliationInfo>
<Affiliation>9CNRS, Univ. Grenoble Alpes, CERMAV, Grenoble, France.</Affiliation>
<Identifier Source="ISNI">0000 0001 2112 9282</Identifier>
<Identifier Source="GRID">grid.4444.0</Identifier>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Launay</LastName>
<ForeName>Charlène</ForeName>
<Initials>C</Initials>
<AffiliationInfo>
<Affiliation>6INRA, UR1268 Biopolymères Interactions Assemblages, Nantes, France.</Affiliation>
<Identifier Source="GRID">grid.460203.3</Identifier>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Margeot</LastName>
<ForeName>Antoine</ForeName>
<Initials>A</Initials>
<AffiliationInfo>
<Affiliation>4IFP Energies Nouvelles, Rueil-Malmaison, France.</Affiliation>
<Identifier Source="ISNI">0000 0001 2159 7561</Identifier>
<Identifier Source="GRID">grid.13464.34</Identifier>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Nishiyama</LastName>
<ForeName>Yoshiharu</ForeName>
<Initials>Y</Initials>
<AffiliationInfo>
<Affiliation>9CNRS, Univ. Grenoble Alpes, CERMAV, Grenoble, France.</Affiliation>
<Identifier Source="ISNI">0000 0001 2112 9282</Identifier>
<Identifier Source="GRID">grid.4444.0</Identifier>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Raouche</LastName>
<ForeName>Sana</ForeName>
<Initials>S</Initials>
<AffiliationInfo>
<Affiliation>2INRA, Aix Marseille Univ., UMR1163, BBF, Biodiversité et Biotechnologie Fongiques, Marseille, France.</Affiliation>
<Identifier Source="GRID">grid.503114.2</Identifier>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Rosso</LastName>
<ForeName>Marie-Noëlle</ForeName>
<Initials>MN</Initials>
<AffiliationInfo>
<Affiliation>2INRA, Aix Marseille Univ., UMR1163, BBF, Biodiversité et Biotechnologie Fongiques, Marseille, France.</Affiliation>
<Identifier Source="GRID">grid.503114.2</Identifier>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Bonnin</LastName>
<ForeName>Estelle</ForeName>
<Initials>E</Initials>
<AffiliationInfo>
<Affiliation>6INRA, UR1268 Biopolymères Interactions Assemblages, Nantes, France.</Affiliation>
<Identifier Source="GRID">grid.460203.3</Identifier>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Berrin</LastName>
<ForeName>Jean-Guy</ForeName>
<Initials>JG</Initials>
<Identifier Source="ORCID">0000-0001-7570-3745</Identifier>
<AffiliationInfo>
<Affiliation>2INRA, Aix Marseille Univ., UMR1163, BBF, Biodiversité et Biotechnologie Fongiques, Marseille, France.</Affiliation>
<Identifier Source="GRID">grid.503114.2</Identifier>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList>
<PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic">
<Year>2019</Year>
<Month>04</Month>
<Day>01</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo>
<Country>England</Country>
<MedlineTA>Biotechnol Biofuels</MedlineTA>
<NlmUniqueID>101316935</NlmUniqueID>
<ISSNLinking>1754-6834</ISSNLinking>
</MedlineJournalInfo>
<KeywordList Owner="NOTNLM">
<Keyword MajorTopicYN="N">Biomass</Keyword>
<Keyword MajorTopicYN="N">Enzymatic degradation</Keyword>
<Keyword MajorTopicYN="N">Filamentous fungi</Keyword>
<Keyword MajorTopicYN="N">Glucose release</Keyword>
<Keyword MajorTopicYN="N">Hydrolysis</Keyword>
<Keyword MajorTopicYN="N">Saccharification</Keyword>
<Keyword MajorTopicYN="N">Water sorption</Keyword>
</KeywordList>
<CoiStatement>The authors declare that they have no competing interests.</CoiStatement>
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<Month>03</Month>
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